DescriptionViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, EvaGreen® dye, as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.

ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step real time RT-qPCR reaction set up
• Equipped with thermostable M-MULV enzyme EvaGreen® dye – highest dye stability and safety
• Good buffer system for excellent amplification efficiency
• Minimal PCR inhibition
• Reliable quantification of low copy number targets
• Minimal PCR inhibition
• Optimal performance for highly sensitive and specific RT-qPCR reaction
• Compatible with most of the real-time PCR platforms

Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Ordering Information

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ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS Taq qPCR Green MasterMix II with ROX (EvaGreen® Dye)

PublicationThis Product Has Been Used In: Jahantigh, M., Mazaheri, M., Moghadam, A.Y., Motalleb, G., Najafi, S., Najafi, S., Pourrahmat, E., Rashki, A., Sabagh, K., Sanadgol, N., Talee, R. (2014).Epidermal growth factor receptor gene expression evaluation in colorectal cancer patients, Indian Journal of Cancer, Vol. 51, No. 3, 358-362 (2014).