GradeMolecular Biology Grade
DescriptionDNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.
Specification
| Appearance | White to off-white crystalline powder; a partially purified, lyophilized powder |
| Source | Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation |
| Unit Definition | One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit. |
StorageStore at -20°C
| Assay | Resuls |
| Activity | >500Kunitz U/mg |
| Loss on Drying | ≤5.0% |
| Residue on Ignition | ≤1.0% |
Ordering Information
| Catalog No | Description | Pack Size |
| PC0704-25mg | Deoxyribonuclease I (DNase I) | 25mg |
| PC0704-50mg | Deoxyribonuclease I (DNase I) | 50mg |
| PC0704-100mg | Deoxyribonuclease I (DNase I) | 100mg |
| PC0704-1g | Deoxyribonuclease I (DNase I) | 1g |
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Deoxyribonuclease I (DNase I)
