DescriptionRibonuclease Inhibitor RNase-free inhibits the activity of RNase A, B, C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit RNase 1, T1, T2, H, U1, U2, CL3 and other enzymes.
Concentrations 40u/μl
Features
- Performs under a wide range of reaction conditions.
- Protects RNA from degradation at temperature up to 55˚C.
- Increase the time RNA can be safely stored.
Assay Conditions 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/ml BSA, 100ng/ml RNase, 0.1mg/ml E.coli [3H]¯RNA, 50mg/ml yeast RNA and 8mM DTT.
Storage Buffer 20mM HEPES-KOH (pH7.5), 50mM KCl, 5mM DTT and 50% glycerol.
Thermal Inactivation 65˚C for 15 minutes
Unit Definition 1u is defined as the amount of ribonuclease inhibitor that inhibits the activity of 5ng of Ribonuclease A by 50%.
Application
- Applied in procedures where RNase contamination constitutes a problem:
- - in vitro transcription.
- - in vitro translation.
- - cDNA synthesis.
- - isolation of mammalian cell fractions that contain mRNA-protein complex.
- - separation and identification of specific ribonuclease activities.
Quality ControlThe absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests. Functionally tested in RNA and cDNA synthesis.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME4309 | Ribonuclease Inhibitor RNase-Free | 2500u |
| ME4310 | Ribonuclease Inhibitor RNase-Free | 4 x 2500u |
DownloadManual
Ribonuclease Inhibitor RNase-Free
PublicationThis Product Has Been Used In: Abdou, H.M., Yousef, M.I., Mekkawy, D.A.E., Al-Shami, A.S. (2016))Prophylactic neuroprotective efficiency of co-administration of Ginkgo biloba and Trifolium pretense against sodium arsenite-induced neurotoxicity and dementia in different regions of brain and spinal cord of rats. Food and Chemical Toxicology. 96. Pp.112-127.Azimpour, S., & Pourtaghi, H. (2016) A Case Report of Fungal Diarrhea in a Preweaned Calf in Iran. International Journal of Enteric Pathogen. 4(2). Azimpour, S., & Pourtaghi, H. (2016) Comparative Evaluation of Conventional and Molecular Diagnostic Methods for Newcastle Disease During and Outbreak in Punjab, Pakistan. Pakistan Journal of Agricultural Sciences.51(3), p. 703-709. Sharifi, K., et al. (2015)The Effect of TNF-α (tumor necrosis Factor alpha) on Expression of MMP9 (matrix metalloproteinase 9) in Human Mesenchymal Bone Marrow Derived Stem Cells). 1st International and 9th National Biotechnology Congress of Islamic Republic of Iran.
