Concentration1-3u/μl5"…AA↓CGTT…3"3"…TTGC↑AA…5"
Reaction Conditions 1X Buffer V5 30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 0.05% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation65°C for 20 minutes.
Ligation / Recutting Assay After 3-fold overdigestion with Acl I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 1.5u of Acl I for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)*Blocked by CpG methylation
Ordering Information
| Catalog No | Description | Pack Size |
| RE1112 | Acl I | 100u |
DownloadsManualAcl I
