Concentration10-30u/μl 5"…G↓GTACC…3" 3"…CCATG↑G…5"

Reaction Conditions 1X Buffer V4 10mM Tris-HCI (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCI, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes.

Ligation / Recutting Assay After 10-fold overdigestion with Acc65I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Acc65I for 16 hours at 37°C.Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent) *Blocked by overlapping dcm¯ methylation (CmCWGG): GGTACCWGG

Ordering Information

DownloadsManualAcc65I {KpnI*}