DescriptionThe GF-1 Blood DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from fresh and frozen anti-coagulated whole blood. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

• Yields up to 20μg of DNA
• No organic-based extraction required
• Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

• Buffer BB
• Wash Buffer 1 (concentrate)
• Wash Buffer 2 (concentrate)
• Elution Buffer
• Proteinase K

Ordering Information

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GF-1 Blood DNA Extraction Kit

Stability Test Report

GF-1 Blood DNA Extraction Kit

PublicationThis Product Has Been Used In: Alhomsi et al. (2020) Assessment of vitamin D-binding protein (DBP) gene polymorphisms and their correlation with multiple sclerosis: a case-control study in a sample of the Syrian population, Egyptian Journal of Medical Human Genetics, 21:32Gorgisen G, Karatas U, Ates C, Oksuz M & Gulacar IM (2020)Association of IRS1 Gly972Arg and IRS2 Gly1057Asp polymorphisms with gastric cancer in Turkish subjects, Oncology Letters, 20:2016-2020Wannapa, Settheetham-Ishida et al. (2020)Genetic Polymorphism of Glutathione S-transferase and Cervical Cancer Susceptibility in Northeastern Thailand, Asian Pacific Journal of Cancer Biology, 5(2): 35-41.Wongpratate et al. (2020)Genetic Polymorphisms of the Human Cytochrome P450 1A1 (CYP1A1) and Cervical Cancer Susceptibility among Northeast Thai Women, Asian Pacific Journal of Cancer Prevention, 21(1):243-248.Yusuf et al. (2020) A preliminary study MUC5B promoter polymorphism and its association with IPF, The Egyptian Journal of Bronchology, 14:18.Panan Kanchanaphum (2018) Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction, . BioMed Research International.Cilingir, O, Ozkan S., Aras, B.D., Erzurumluoglu, E., Kutlay, O., Akinci, M., Emir, B., Afagh, A., Artan, S., (2017) Association of functional RAGE gene polymorphisms with Parkinson’s disease in a Turkish cohort. Biomedical Research 2017; 28 (19): 8454-8460Piratae, S., Sae-chue, B., Sukumolanan, P., Phosri, A. (2017).). Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand, . , Asian Pacific Journal of Tropical Disease , Vol. 7, No. 4, 233-236 (2017).. Rani, A., Nawaz, S.K., Irfan, S., Arshad, M., Bashir, R., Shaheen, N. (2017). Role of MyD88-adaptor-like gene polymorphism rs8177374 in modulation of malaria severity in the Pakistani population, The Brazilian Journal of Infectious Diseases, Vol. 21, No. 4. (2017). Abajy, M.Y., Ibrahim, A., Almohsen, J.A.(2016). Development of New AS-PCR based Analytical Approach for detecting the Single Nucleotide Polymorphism of AGTR.1 gene, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, No. 7 (2016). Babker,A.M.A.A., Gameel, F.E.M.H. (2016).Methylenetetrahydrofolate Reductase C677T Polymorphism in Sudanese Women with Recurrent Spontaneous Abortions, Kuwait Medical Journal, Vol. 48, No. 2, 100-104 (2016).Zahri, M.K., Emilia, A., Rawi, R.I.M., Taib W.R.W., Sani, A.I., Baig, A.A. (2016)Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity. Meta Gene. 10. Pp..39-44. Nawaz, S.K., et al. (2015) Role of S180L Polymorphism in Etiology of Malaria Caused by Plasmodium falciparum in a Small Group of Pakistani Population. Bosnian Journal of Basic Medical Sciences. 15(4), p.20-23. Nawaz, S.K., Rani, A., Yousaf, M., Noreen, A., Arshad, M. 2015. Genetic etiology of coronary artery disease considering NOS 3 genevariant rs1799983. Vascular. 23(3) pp.270-276Naila, R., et al (2013)Elevated genetic deletion of GSTT1 in Pakistani population JÖKULL Journal63(12).Skalar, C., Gurbuz, E., Kalay, N., Kaya, M.G.(2013). Higher frequency of 4977574 (the G Allele) on Chromosome 9p21.3 in Patients with Myocardial Infarction as Revealed by PCR-RFLP Analysis (2013) The Tohoku Journal of Experimental Medicine, Vol 230, No. 3, 171-176 (2013).Amer, H.M. et al. (2011) An Optimized Polymerase Chain Reaction Assay to Identify Avian Virus Vaccine Contamination with Chicken anemia Virus Journal of Veterinary Diagnostic Investigation. Sage Journals. 23, p. 34-40.